Melanoleuca chifengense2021

Melanoleuca chifengense X.D. Yu & H.B. Guo, sp. nov.2021

MycoBank: 838026

Type:China. Inner Mongolia: Chifeng City, Linxi County, Xinlin Town, Dau ran Village, alt. 1200 m, 44.00°N, 118.07°E, 21 Aug 2017, H.B. Guo (SYAU-FUN GI-059).

Description:Pileus 30–60 mm diam., flat at first, becoming depressed at disc when mature, margin sometimes cracking, surface glabrous, grey to yellowish-grey greyish-brown  at centre, often darker at margin. Lamellae crowded, adnate to decurrent, white to yellowish-white , 2.5–3.0 mm broad, with lamellulae, edge entire. Stipe cylindrical, 20–35 mm long × 2–5 mm diam., central, broadened at base, solid, surface grey to yellowish-grey at first , becoming yellowish-brown with age or after touching, striate, often with whitish ba sal tomentum. Pileus context up to 10 mm thick near stipe attachment, thin at margin, yellowish-grey, grayish brown to yellowish brown in stipe cortex, up to brown in stipe base. Odour none, taste mild. Spore print white.

Sexual morph：Basidiospores (90/6/2) 7.0–8.5 (9.0) × 4.0–6.2(6.5) μm, av. 7.5 × 5.2 μm, Q = 1.40–1.45(1.50), ellipsoid, hyaline, amyloid, ornamentation verruculose, with irregu lar warts, sometimes with ridges. Basidia (20) 23–29 (30) × (7.0) 7.5–9.0 (10.0) μm, av. 26 × 8.5 μm, clavate, 4-spored, sometimes 2-spored, subhyaline. Hymenial cys tidia absent, lamella edge sterile. Hymenophoral trama 42–85 μm broad, regular with thin-walled hyphae 5.5–16.5 μm diam., hyphae not pigmented. Subhymenium poorly developed. Pileipellis a cutis of numerous repent branched hyphae, 5.5–7.5 μm wide, thin-walled. Stipitipellis hyphae 3.5–8.0 μm diam., thin-walled, hyaline. Caulocyst idia absent. Clamp connections absent.

Asexual morph: Undetermined:

Culture: Undetermined:

Habit：On soil or meadow outside of a forest, often on the roadside near a forest. 

Distribution：Known from north-eastern China.

GenBank Accession: ITS MW258678,nrLSU MW258688,RPB2 MW28154;

ITS MW258679,nrLSU MW258689,RPB2 MW281547;

Notes: Undetermined:

Reference: [1]SX, XD, FF, Lin, & Zhang. (2014). Two new species of melanoleuca (agaricales, basidiomycota) from northeastern china, supported by morphological and molecular data. Mycoscience, 55(6), 456-461.

A macroscopic habit B surface of basidiospores. Scale bars: 1 cm (A); 5 μm (B)

A–C Melanoleuca chifengense (holotype, SYAU-FUNGI-059) A basidiospores B, C basidia and basidioles