Melanoleuca griseoflava 2021

Melanoleuca griseoflava X.D. Yu & H.B. Guo, sp. Nov2021

MycoBank:838027

Type: China. Liaoning Province: Shenyang City, Tianzhu Mountain, on the soil in woods, 31 Aug 2019, X.D. Yu (holotype: SYAU-FUNGI-062).

Description：Pileus 35–60 mm diam., flat at first, then gradually depressed, mar gin slightly inflexed when mature, surface fibrillose, greyish-brown , be coming deep yellow  at centre. Lamellae crowded, adnexed to adnate, white, 2.5–3.0 mm broad, with lamellulae, edge entire. Stipe cylindrical, 30–50 mm long × 3–5 mm diam., central, somewhat broadened at the base, fibrous, expanded at base, solid, surface yellowish-grey to greyish at first, becoming yellowish brown  with age, striate, with whitish basal tomentum. Pileus context up to 10 mm thick near stipe attachment, thin at margin, greyish-yellow to yellowish-grey , yellowish-grey  in stipe cortex, whitish in stipe base. Odour none, taste mild. Spore deposit white.

Sexual morph：Basidiospores (234/10/8) (5.0) 6.0–7.2 (8.0) × 4.0–5.0 (6.0) μm, av. 6.5 × 4.5 μm, Q = (1.30)1.45–1.55 (1.60), ellipsoid, hyaline, amyloid, ornamentation verruculose, warts with ridges, almost reticulate. Basidia (18) 20–25 (28) × (4.0) 5.0–6.5 (7.0) μm, av. 22 × 6.0 μm, clavate, 4-spored, occasionally 2-spored, hya line. Cheilocystidia (40) 45–55 (60) × (6.0) 8.0–12.0 (15.0) μm, fusiform, thin walled, with encrusted crystals at apex, abundance. Pleurocystidia scattered, similar to cheilocystidia. Hymenophoral trama 90–150 μm broad, regular with thin-walled hyphae 3.0–14.0 μm diam., hyphae not pigmented, lamella edge sterile, Subhy menium poorly developed. Pileipellis a cutis of numerous repent branched hyphae 7.5–10.5 μm wide, thin-walled, pigmented with light violet. Stipitipellis hyphae 3–10.0 μm diam., smooth, thin-walled, pigmented. Caulocystidia of two types of cells, (1) 40–90 × 6.0–10.0 μm, fusiform, thin-walled, some with encrusted crystals at apex, similar to cheilocystidia; (2) 30–40 × 7.0–10.0 μm, clavate, thin-walled, without crystals. Clamp connections absent.

ASexual morph：Undetermined:

Culture: Undetermined:

Habit：Solitary, saprotrophic on the soil, on the grass, on roadsides, in woods.

Distribution：Known from north-eastern China.

GenBank Accession: ITS MW258680，nrLSU MW258685，RPB2 MW281544；ITS MW258681；ITS MW258682；ITS MW258683，nrLSU MW258686，RPB2 MW281548；

Notes: Undetermined:

Reference: [1]SX, XD, FF, Lin, & Zhang. (2014). Two new species of melanoleuca (agaricales, basidiomycota) from northeastern china, supported by morphological and molecular data. Mycoscience, 55(6), 456-461.

A macroscopic habit B, C surface of basidiospores D hymenial cystidia with encrusted crystals at apex. Scale bars: 1 cm (A); 5 μm (B, C); 10 μm (D)

D–G Melanoleuca griseoflava (holotype, SYAU-FUNGI-062) D basidia and basidioles E caulocystidia F basidiospores G cheilocystidia