Coniochaeta mongoliae2021

Coniochaeta mongoliae H. L. Si & Y. M. Su, sp. nov. 2021

MycoBank: 839389

Holotype. China, the Inner Mongolia Autonomous Region, Chifeng City, 44°13'46"N, 118°44'57"E, ca. 1500 m elev., isolated from the medulla of Ramalina sinensis, 11 Oct 2019, H. L. Si, CS-09 (HMAS 350270, holotype), ex-type living culture, CGMCC

Morphological description :

Asexual morph: Colony on PDA after 8 d, hyphae hyaline, multi-guttulate, septate, smooth-walled, often with hyphal strands consolidating to form bundles; conidiophores short or absent; conidiogenous cells hyaline, flask or acicular in shape, measuring 3.3–12.5 × 1.6–5.1 μm (x = 6.6 × 2.9 μm, n = 50) (Fig. 4c, d); conidia hyaline, ̅ smooth-walled, ellipsoidal, 3.3–8.4 × 0.6–1.9 μm (x = 4.8 × 1.3 μm, n = 50) (Fig. 4g and h); chlamydospore solitary or in short chains, hyaline, thick-walled, elongate ellipsoidal or almost globose in shape, measuring 2.7–6.7 × 2.6–5.4 μm (x = 4.6 × 3.8 μm, ̅ n = 50) (Fig. 4e, f). 

Sexual morph unknown.

Culture characteristics. The optimal temperature for growth is 25 °C. No growth was detected at 5 °C and 35 °C. Colonies on PDA after 8 d at 25 °C were white to light pink in colour, circular, flat, dense, partially immersed in the medium, the centre of the colony is rough, forming radial grooves.

Habitat: , isolated from the medulla of Ramalina sinensis,

Distribution: China, the Inner Mongolia Autonomous Region,

GenBank:  lsu MW077646; its MW077645.

Notes:In the phylogenetic tree using the concatenated dataset, Coniochaeta mongoliae sp. nov. clustered in a clade that included C. sinensis sp. nov., C. vineae and C. fasciculata, but with low statistical support. Moreover, these four species have substantial morphological differences (for details, see the notes for C. sinensis sp. nov.

Reference: [1] Limkaisang, S. ,  Kom-Un, S. ,   Furtado, E. L. ,  Liew, K. W. ,  Salleh, B. , &  Sato, Y. , et al. (2005). Molecular phylogenetic and morphological analyses of oidium heveae, a powdery mildew of rubber tree. Mycoscience, 46(4), 220-226.

Morphological characters of Coniochaeta mongoliae sp. nov. (HMAS 350270) a, b cultures on PDA from the surface and reverse, c, d conidiogenous cells e, f chlamydospores g, h conidia. Scale bars: 10 μm