Pseudocapulatispora fragrantis C.F. Liao & Doilom, sp. nov. 2021
MycoBank Index Fungorum number: IF558530, Facesoffungi number: FoF 09897
Holotype: KUMCC 21-0012.
Morphological description
Sexual morph：Ascomata 220–377 × 162–325 μm (x̅= 303 × 268 µm, n = 30), immersed to erumpent, appearing as black spots or short black bands, coriaceous, solitary to scattered, globose to subglobose, base flattened, pyriform, light brown to black. Ostioles 74–195 × 17–66 μm (x̅= 125 × 32 µm, n = 30), black short bands, crestlike, elongate and laterally compressed, carbonaceous, filled with hyaline periphyses. Peridium 7–16 μm wide (x̅ = 12 µm, n = 30), composed of 3–5 layers, brown to hyaline cells of textura angularis, based thin towards to the thickened ostiole. Hamathecium comprising 1.2–2.8 μm wide (x̅= 1.8 µm, n = 30), dense, filiform, septate, branching pseudoparaphyses, short cylindrical to ovoid at the base. Asci 58–127 × 8–15 μm (x̅ = 99 × 12 µm, n = 30), 8-spored, bitunicate, fissitunicate, cylindric-clavate, straight to slightly curved, apically rounded, with short, with furcate pedicel and ocular chamber. Ascospores 29–36 × 4–8 μm (x̅= 32 × 5 µm, n = 30), overlapping biseriate, hyaline, aseptate when young, becoming one-septate when mature, constricted at the septa, broad fusiform, tapering towards the ends, straight to slightly curved, with 1–4 guttules when young, becoming two large guttules when mature, smooth-walled, with a broad sheath; sheath drawn out from apices of ascospores with polar appendages, 16–46 ×8–14 μm (x̅= 26 × 10 µm, n = 30), with globose drops at their apices.
Asexual morphs:
Cultures: Ascospores germinating on PDA within 24h, germ tube produced from both ends. Colonies on PDA reaching 12–15 diam, after 2 weeks at room temperature (25±2℃),medium dense, raised at the center, circular, floccose to fluffy, velvety, brownish-greyish in the middle, whitish towards the margin; reverse brown to yellowish-brown, no pigment produced.
Habitat: on dead stem of Heteropanox fragrans.
Distribution: China, Yunnan Province, Kunming.
GenBank Accession: (SSU) MZ475353, (LSU) MZ475352, (ITS) MZ475354 and (tef-1) MZ463059.
Notes: Pseudocapulatispora fragrantis (KUMCC 21-0012) clustered with P. longiappendiculata (MFLUCC 17-1452, ex-type, MFLUCC 17-1457), and P. clematidis (MFLUCC 17-2063, ex-type), but in separate branches with 100% ML and 1.00 PP bootstrap support (Fig. 8). Pseudocapulatispora fragrantis differs from the holotype of P. longiappendiculata in having larger ascomata (303 × 268 vs 240 × 135 μm), thinner peridium (7–16 vs 15–25 µm), larger ascospores (32 × 5 vs 26 × 7.5 µm), with wider polar appendages (26 × 10 vs 25 × 4.5 μm). While, it differs from the holotype of P. clematidis in having longer ostiole (125 × 32 vs 107 × 66 μm), larger ascospores (32 × 5 vs 25 × 9 μm) and longer polar appendages (26 × 10 vs 19 × 4 µm). Thus, we introduce Pseudocapulatispora fragrantis as a new species based on morphology and phylogenetic evidence.
Reference: Hyde KD, Suwannarach N, Jayawardena RS, Manawasinghe IS, Liao CF, Doilom M, Cai L, Zhao P, Buyck B, Phukhamsakda C, Su WX, Fu YP, Li Y, Zhao RL, He MQ, Li JX, Tibpromma S, Lu L, Tang X, Kang JC, Ren GC, Gui H, Hofstetter V, Ryoo R, Antonín V, Hurdeal VG, Gentikaki E, Zhang JY, Lu YZ, Senanayake IC, Yu FM, Zhao Q, Bao DF 2021 – Mycosphere notes 325-344 – Novel species and records of fungal taxa from around the world. Mycosphere 12(1), 1101–1156, Doi 10.5943/mycosphere/12/1/14
Pseudocapulatispora fragrantis (KUN–HKAS 107635, holotype). a−c Appearance of ascomata on substrate. d, e Section through ascoma. f Peridium. g Ostiole. h Pseudoparaphyses stained in Congo red. i–k Asci stained in Congo red. l–n Ascospores stained in Congo red. o Ascospore in Indian Ink. p Germinated ascospore. q, r Culture characteristics on PDA. Scale bars: e = 500 μm, f, h = 200 μm, g, i–o = 50 μm, k = 20 μm.
