Claviformispora phyllostachydis 2020

Claviformispora phyllostachydis X. L. Xu & C. L. Yang 2020

Index Fungorum identifier: IF557396

Holotype: China, Sichuan Province, Ya’an City, Yucheng Distinct, Kongping Township, alt. 1133 m, 29°50.14'N, 103°03'E, on dead branches of Phyllostachys heteroclada Oliv. (Poaceae), 11 December 2016, C. L. Yang and X. L. Xu, YCL201612002 (SICAU 

16-0007, holotype; MFLU 18-1217, isotype), ex-type living culture, SICAUCC 160004 = MFLUCC 18-1230.

Morphological description

Sexual morph: Stromata solitary comprising elliptical areas or aggregated in large black areas, slightly raised with slit-like openings presenting on host surface. Ascomata 120–240 μm high × 220–490 μm diameter (x¯  = 189 × 345 μm, n = 20), perithecial, immersed, central, papillate ostiole with periphyses, oval-globose in section, the cells between the perithecia are composed with tissue of stromata and host. Peridium 20–40 μm wide (x¯  = 33 μm, n = 10), outer cells merging with the host tissues, composed of pale to dark brown cells of textura angularis. Hamathecium comprising hyaline, hypha-like, septate paraphyses, occasionally branched, longer than asci, wider at the base, 2–4 μm diameter (x¯  = 2.7 μm, n = 20) tapering towards the apex, 0.78– 1.20 μm diameter (x¯   = 0.98 μm, n = 20). Asci 90–160 × 9–15 μm (x¯  = 118 × 13 μm, n = 20), 8-spored, cylindrical to cylindric-clavate, unitunicate, short pedicellate, apically rounded, with a massive, doughnut-shaped, refractive, J- reaction, apical ring. Ascospores 35–50 × 5.7–8.6 μm (x¯  = 45.7 × 7.0 μm, n = 40), overlapping uniseriate or 2-seriate, claviform typically with a thin pedicel, aseptate, hyaline, straight or slight curved, without appendage and septum-like bands, guttulate when maturity. Asexual morph: Undetermined.

Culture characters. Ascospores germinated on PDA within 12 hours at both ends. Colonies on PDA reaching 5 cm diameter after 7 days at 25 °C, white to grey with strong radiations outwards on forward side. Colonies became dark brown and black on the reverse after a long time of cultivation. The hyphae are septate, branched, smooth.

Habitat: on dead branches of Phyllostachys heteroclada Oliv.

Distribution: China.

GenBank Accession: LSU : MT232720; ITS: MT232736; SSU: MT232735; TEF: MT240855

Notes: 

Reference: Xu X-L, Yang C-L, Jeewon R et al. (2020) Morpho-molecular diversity of Linocarpaceae (Chaetosphaeriales): Claviformispora gen. nov. from decaying branches of Phyllostachys heteroclada. MycoKeys 70: 1–17. https://doi.org/10.3897/mycokeys.70.54231

Claviformispora phyllostachydis (SICAU 16-0007, holotype) a, b Stromata on host substrate c section through ascoma with ascomata d ostiole with periphyses e peridium f paraphyses g–j asci k–o ascospores p germinated ascospore q, r colony on PDA after 7 days. Scale bars: 2 mm (a), 500 μm (b), 100 μm (c), 20 μm (d, e), 10 μm (f–p).