Diaporthe lenispora Y.Y. Chen, A.J. Dissanayake and Jian K. Liu sp. nov. 2020
Index Fungorum number: IF IF557392; Facesoffungi Number: FoF07857; MycoBank Number: MB836214
Holotype: China, Guizhou Province, Guizhou Province, Suiyang broad water nature reserve, on decaying branch, April 2018. Y.Y. Chen (HKAS 107538, holotype), ex-type living culture CGMCC 3.20101 = GZCC 19-0145; ibid., Xingyi Wanfenglin, saprobic on decaying woody host, June 2019, Y. Y. Chen (GZAAS 19-2066, paratype), living culture GZCC 19-0343; ibid., saprobic on decaying branch, June 2018. Y.Y. Chen, GZAAS 19-2075, living culture GZCC19-0351.
Morphological description
Saprobic on decaying woody branch. Sexual morph: Ascomata 435–510 µm diam, black, globose to conical, scattered irregularly, immersed in host tissue with elongated, long necks protruding through substrata. Asci 44–53 µm × 9–10 µm (x = 48 × 9, n = 30), 8-spored, unitunicate, sessile, elongate to clavate. Ascospores 10–12 × 2–3 µm (x = 11 × 2.5, n = 50), hyaline, two-celled, often 4-guttulate, with larger guttules at centre and smaller one at ends, elongated to elliptical. Asexual morph: Not observed.Culture characteristics: Colonies covering entire PDA Petri dishes after 10 d at 25 ◦C producing abundant white aerial mycelium, reverse early yellow and turned to fuscous black.
Habitat: on decaying branch
Distribution: China
GenBank Accession:
Notes: In the combined phylogenetic tree, Diaporthe lenispora groups in a distinct clade with maximum support (ML/MP/BI = 100/100/1.0) and it appears to be most closely related to D. vawdreyi (Figure 1). Diaporthe lenispora can be distinguished from D. vawdreyi based on ITS, tef and tub loci (19/539 in ITS, 56/467 in tef and 23/453 in tub), cal and his gene regions are unavailable for D. vawdreyi. We are not able to compare the morphology of D. lenispora and D. vawdreyi as the latter has no reported sexual morph [74].
Reference: Asha J. Dissanayake, Ya-Ya Chen and Jian-Kui (Jack) Liu
Diaporthe lenispora (HKAS 107538, holotype). (a,b) Ascomata on host surface. (c) Ostiole. (d,e) Section of ascomata. (f) Immature ascus. (g–i) Mature asci. (j,k) Ascospores. (l) 10 days old culture on PDA from above. (m) 10 days old culture on PDA from reverse. Scale bars: (c) = 100 µm, (d,e) = 50 µm, (f–j) = 10 µm, (k) = 5 µm.
