Diaporthe ellipsospora Y.Y. Chen, A.J. Dissanayake and Jian K. Liu sp. nov. 2020
Index Fungorum number: IF557389; Facesoffungi Number: FoF07854; MycoBank Number: MB836175
Holotype: China, Guizhou Province, Xingyi Wanfenglin, Saprobic on decaying branch,
June 2019, Y.Y. Chen (HKAS 107535, holotype), ex-type living culture CGMCC 3.20099 = GZCC 19-0231; ibid, (GZAAS 19-2061, paratype), living culture GZCC 19-0342; ibid, Maolan Nature Reserve, saprobic on decaying woody host, June 2019, Y. Y. Chen, GZAAS 19-2080, living culture GZCC 19-0357.
Morphological description
Saprobic on decaying branch. Sexual morph: Ascomata 380–430 µm diam, black, globose to irregular, scattered on dead twigs, immersed in host tissue, protruding through substrata. Paraphyses up to 100 µm long, rarely present, hyaline, smooth, 1–3-septate, cylindrical with obtuse ends, extending above conidiophores. Asci 40–47 ×7–8.5 µm (x = 43 × 7.5, n = 30), 8-spored, unitunicate, sessile, elongate to clavate. Ascospores 8.5–13 × 2.5–3.5 µm (x = 10.1 × 3.3, n = 50), hyaline, elongated to elliptical, two-celled, often 4-guttulate, with larger guttules at center and smaller ones at ends. Asexual morph: Not observed.Culture characteristics: Colonies on PDA fast growing, covering entire PDA Petri dish after 8 d at 25 ◦C. White, aerial mycelium turning grey at edges of plate, reverse yellowish pigmentation developing in centre.
Habitat: on decaying branch
Distribution: China
GenBank Accession:
Notes: Diaporthe ellipsospora formed an independent clade (Figure 1) and is phylogenetically distinct from D. aquatica in a well-supported clade (ML/MP/BI = 96/100/1.0). Diaporthe ellipsospora can be distinguished from D. aquatica (IFRDCC 3051) only based on ITS locus (17/539) since other gene sequences (tef, tub, cal and his) are unavailable for D. aquatica. Diaporthe ellipsospora can be morphologically differentiated from D. aquatica as the latter has long necks up to 2250 µm [58].
Reference: Asha J. Dissanayake, Ya-Ya Chen and Jian-Kui (Jack) Liu
Diaporthe ellipsospora (HKAS 107535, holotype). (a,b) Ascomata on host surface. (c) Section of an ascoma. (d) 8 days old culture on PDA from above. (e) 8 days old culture on PDA from reverse. (f) Immature ascus. (g–k) Mature asci. (l–p) Ascospores. (q) Paraphyses. Scale bars: (c) = 100 µm, (f–k) = 10 µm, (l–q) = 5 µm.
