Nemania changningensis2021

Nemania changningensis Y.H. Pi & Q.R. Li, sp. nov.2021

MycoBank No: 840087

Holotype: GMBC0056, ex-type living culture; KUN-HKAS 112668, isotype

Morphological description

Sexual morph: Stromata effused-pulvinate, confluent into irregularly elongated compound stromata, up to 18–35 mm long × 2–4 mm wide × 0.3–0.5 mm high, irregularly lobed, plane or with inconspicuous perithecial mounds and sloping margins; surface covered with white tissue, persistent layer, with blackish-grey carbonaceous sub-surface showing through in places; the tissue beneath the perithecial layer inconspicuous, greyish-white in places, the underlying wood blackened; mature stromata lacking KOH extractable pigments. Perithecia 0.45–0.6 mm diam. × 0.4–0.55 mm high, subglobose to depressed-spherical. Ostioles slightly higher than stromatal surface and with openings papillate, often surrounded by white tissue, inconspicuous, black, without encircling disc. Asci 100–140 × 7–10 μm (av. = 111 × 8.5 μm, n = 30), 8-spored, unitunicate, cylindrical, short-stipitate, the spore-bearing parts 70–90 µm long, the apical apparatus of immature asci blue in Melzer’s Reagent, but not blue in mature asci. Ascospores 10–13 × 4–6.5 μm (av. = 11.5 × 5.5 μm, n = 30), uniseriate unicellular, smooth, light brown, slightly inequilateral, with broadly rounded ends, inconspicuous or lack a germ slit; perispore indehiscent in 10% KOH. 

Asexual morph: Undetermined.

Cultures: The colony grows slowly on the PDA with a diameter of 4.5 cm after 2 weeks at 25 °C. The colony on the surface is white, thick and flat in the middle, edges are shallow, irregular bands and rosettes. Colony reverse is orange and intermediate colour darker. Not sporulating on OA nor on PDA.

Habitat: on dead wood, 

Distribution: . China, Yunnan Province, Changning County, Lancang River Nature Reserve (25°01'30.36"N, 99°35'30.53"E, altitude: 2586 m),

GenBank Accession: its  MW851875; rpb2 MW836061; tubulin  MW836027 ;catin MW836042.  Its MW851876; rpb2  MW836062; tubulin  MW836028 ; catin MW836043.

Notes: In the phylogenetic analyses, N. changningensis is on a separate branch and grouped with N. caries with high support values (100% ML, 1 BYPP, Fig. 1). In term of ascospores dimension, N. changningensis resembles N. caries, but differs in the perithecia of N. caries (obovoid, 0.3–0.6 mm diam. × 0.5–0.7 mm high), in the surface not covered with white tissue and in its apical apparatus of mature asci bluing in Melzer’s Reagent (Miller 1961; Ju and Rogers 2002).

Reference: [1] Song, Y. J. , &  Li, S. Q. . (2008). A taxonomic study of chinese nematogmus species (araneae, linyphiidae). Organisms, diversity, & evolution(4), 8.

Nemania changningensis (GMB0056, holotype) A type material B, C stromata on the surface of host D transverse sections of stromata E longitudinal section of stroma F–H asci with ascospores I pigments in 10% KOH J, K asci apical apparatus (stained in Melzer’s Reagent) L, M ascospores N, O colonies on PDA (N-upper, O-lower). Scale bars: 0.5 mm (C–E); 10 μm (F–H, J–