Spodocybe rugosiceps 2021

Spodocybe rugosiceps Z. M. He & Zhu L. Yang, sp. nov.2021

MycoBank No: 839052

Holotype: China. Yunnan Province: Kunming City, near Yeya Lake, at 25.136658°N, 102.873027°E, alt. 2000 m, 11 Aug 2020, Z. M. He 72 (KUN-HKAS 112563, holotype).

Morphological description

Sexual morph: Basidiomes small, clitocyboid. Pileus 0.5–2 cm in diam, at first near ly applanate, then concave; surface dry and rugose, gray-brown to gray-black in the center and gray-brown or gray towards margin; center often slightly umbonate; margin straight and undulating; context thin and white to cream. Lamellae deeply decurrent, white (1A1) to cream, thin (up to 2 mm high), crowded, sometimes forked and intervenose. Stipe 2.5–6 × 0.2–0.4 cm, central, narrowly cylindrical to subcylindrical, sometimes flexuous, hollow; surface dry and nearly smooth, concolorous with pileus; context white.

    Basidiospores [60/3/3] 5–6 (6.5) × (2.5)3–3.5(4) μm, Q = (1.38)1.55–1.95(2), Qm = 1.73 ± 0.14, elongate, colorless, hyaline, smooth, thin-walled, inamyloid. Ba sidia 20–24 × 5–6 μm, clavate, 4-spored, colorless, hyaline, thin-walled; sterigmata up to 4 μm long; ratio of basidia to basidiospore length values about 3–5. Cystidia absent. Lamellar trama subregular; hyphae colorless, hyaline, cylindrical, thin-walled, 3–10 μm wide. Pileipellis a cutis, but in places upright or trichodermial in appearance, made up with thin-walled cylindrical hyphae 3–9 μm wide. Stipitipellis a cutis, composed of thin-walled cylindrical hyphae 3–10 μm wide. Clamp connections present in all parts of basidiome.

Asexual morph: Undetermined

Culture characteristics:

Habitat: Gregarious or caespitose, growing sapro trophically in forest litter, often under conifers, on the ground.

Distribution: Known from subtropi cal zone of Yunnan, China.

GenBank Accession: 

ITS MW762883; nrLSU MW763008; rpb2 MW789187; rpb1 MW789171; tef1-α  MW773447; atp6 MW789202

ITS MW762884; nrLSU MW763009; rpb2 MW789188; rpb1 MW789172; tef1-α  ; atp6 MW789203

ITS MW762885; nrLSU MW763010; rpb2 MW789189; rpb1 MW789173; tef1-α MW773448; atp6 MW789204

ITS MW762886; nrLSU MW763011; rpb2 MW789190; rpb1 MW789174; tef1-α MW773449; atp6 MW789205

ITS MW762887; nrLSU MW763012; rpb2 MW789191; rpb1 MW789175; tef1-α MW789159; atp6 MW789206

ITS MW762888; nrLSU MW763013; rpb2 MW789192; rpb1 MW789176; tef1-α MW789160; atp6 MW789207

Notes:

Reference: [1] He, Z. , &  Yang, Z. . (2021). A new clitocyboid genusspodocybeand a new subfamily cuphophylloideae in the family hygrophoraceae (agaricales). MycoKeys, 79, 129-148.

Figure 4. Basidiomes of described Spodocybe species. A, B Spodocybe rugosiceps (KUN-HKAS 112563, KUN-HKAS 112562, respectively). Scale bars: 1 cm. 

Figure 5. Microscopic features of Spodocybe rugosiceps (KUN-HKAS 112563, holotype) a basidiospores b basidia c pileipellis. Scale bars: 10 μm.