Typhrasa rugocephala2021

Typhrasa rugocephala J.Q Yan & S.N. Wang sp. nov2021

MycoBank: 838483

Holotype：China. Baishanzu National Nature Reserve, Zhejiang Province, 27.734233°N, 119.186943°E, 1184 m alt. 28 June 2020, Sheng-Nan Wang, HFJAU1467.

Description：

Sexual morph：Pileus 35–55 mm, spreading hemispherically to oblate with a slight umbo, surface with distinct ridge-like folds, hygrophanous, reddish-brown , pale brown  at the margin, drying tawny , striate, some times faintly, at margin. Veil distinct, fibrous or fluffy, white , markedly appen diculate at margin, falling off easily. Context thin and fragile, hygrophanous, about 2.5 mm at the centre. Gills 5.0–7.0 mm broad, moderately close; when young, dirty white, becoming cinnamon with a white edge; two types of long gills arranged at intervals: A: adnate to slightly decurrent, B: emarginate- ad nexed. Stipe 40–60 mm long, 5.0–10 mm thick, white, hollow, pulverulent at apex, with fibrils and fluffy from pellicular veil remnants below, falling off easily. Spores (6.5)6.8–7.9(8.3) × 4.5–5.2(5.4) μm, Q = (1.3)1.4–1.7, ellipsoid to ob long-ellipsoid, profile flattened on one side, 4.1–5.0(5.2) μm broad, smooth, red dish-brown  in water, yellow-brown  in 5% KOH or 10% NH3 ·H2 O, becoming darker in 5% KOH, germ pore small and indis tinct, 1–2 guttulate, inamyloid. Basidia 18–23 × 7.0–8.0 μm, 4-spored, clavate, hya line. Pleurocystidia 42–68 × 13–17 μm, thin-walled, fusiform, apex obtuse to suba cute, rarely cystiform with a short rostrum, with one or two large internal oily drops, oily drops colourless and distinct or indistinct in 5% KOH, glassy-yellow  and very distinct in Melzer’s Reagent. Cheilocystidia scanty, 33–48 × 10–15 μm, similar to pleurocystidia, few and scattered, mix with pyriform or clavate, 21–39 × 11–13 μm-sized cells. Trama of gills consisting of parallel hyphae. Pileipellis a 2–3 cells deep layer of subglobose or pyriform cells which are 18–32 μm wide. Veil com posed of 5.4–8.4 μm-broad hyphae, thin-walled and fawn hyphae in 5% KOH. Clamps rare, but observed in trama of gills, hyphae of stipe and at the base of the basidia and cystidia.

Asexual.morph:Undetermined；

Culture: Undetermined；

Habitat: Undetermined；

Distribution：Saprotrophic, solitary or gregarious on soil or humus in broad-leaved forests.

GenBank Accession: ITS MW466541，LSU MW466546，tef-1α MW475282；

ITS MW466542，LSU MW466547，tef-1αMW475283；ITS MW466543, LSU MW466548;

Notes: Undetermined；

Reference: [1] Wang, S. N. ,  Hu, Y. P. ,  Chen, J. L. ,   Qi, L. L. , &  Yan, J. Q. . First record of the rare genus typhrasa (psathyrellaceae, agaricales) from china with description of two new species. MycoKeys, 79, 119-128.

a–d basidiomata e basidiospores f basidia g pleurocystidia h cheilocystidia. Scale bars: 20 mm (a–d); 10 μm (e–h).