Diaporthe zaofenghuang 2021

Diaporthe zaofenghuang X.H. Wang & G.P. Wang, sp. nov.2021

MycoBank: 838501

Holotype HMAS 249835, cul ture ex-holotype CGMCC3.20271 = TZFH1); ibid., culture TZFH3.

Description：

Sexual morph: Undetermined:

Asexual morph：on alfalfa stems. Pyc nidial conidiomata conical, yellowish translucent conidial drops exuded from ostioles, 650–1430 μm diam. Conidiophores fasciculate, hyaline, long cylindrical, straight or slightly curved, apex pointed, 13.7–20.9 × 1.8–2.7 μm. Conidiogenous cells phialidic, cylindrical. Alpha conidia hyaline, aseptate, ellipsoidal, biguttulate, rounded at one end, slightly apex at another end, 5.3–7.5 × 2.9–3.7 μm (mean = 6.0 ± 0.6 × 3.1 ± 0.3 μm, n = 50). Beta and gamma conidia not observed.

Culture：Cultures incubated on PDA at 25 °C in cycle of light/ darkness, growth rate 8.5 mm per day. Colonies initially white on surface, producing black pigment from center of medium and expanding outwardly after 5–7 d. On PDA, edge of colony petal-like, irregular; on OA, edge relatively flat.

Habit：Undetermined:

Distribution：China

GenBank Accession: Undetermined:

Notes：Two isolates representing D. zaofenghuang form a well-supported clade (1/100/100) and appear to be most closely related to D. penetriteum. Diaporthe zaofen ghuang can be distinguished from D. penetriteum based on ITS, HIS, and TUB loci (10/578 in ITS, 45/520 in HIS, and 7/525 in TUB). Morphologically, D. zaofeng huang differs from D. penetriteum in having larger conidiomata (D. zaofenghuang = 653–1433 μm vs D. penetriteum = 180–490 μm) and alpha conidia (D. zaofenghuang = 6.0 ± 0.6×3.1 ± 0.3 μm vs D. penetriteum = 5.0 ± 0.3 × 2.2 ± 0.2 μm). Addition ally, D. penetriteum produces two types of conidia, but D. zaofenghuang produces only alpha conidia (Gao et al. 2016)

Reference: Undetermined

(A, B) and OA (C, D), respectively E conidiomata on alfalfa stems F conidioma G conidiophores H alpha conidia I–J section view of conidioma. Scale bars: 2 mm (E); 200 μm (F); 20 μm (G, I); 10 μm (H, J).