Spathaspora parajiuxiensis 2020

Spathaspora parajiuxiensis C.Y. Chai & F.L. Hui, sp. nov.2020

MycoBank 836447

Holotype: China, Yunnan Province, Honghe Prefecture, Luxi County, in rotting wood in Jiuxi Mountain Forest Park, July 2016, R.C. Ren & L. Zhang (holotype, NYNU 16747T preserved in a metabolically-inactive state), ex-holotype: CICC 33162; CBS 14691.

Morphological description:

In YM broth after 3 days at 25 °C, cells are ovoid to elongate (3.5–4 × 7–15 μm) and occur singly or in pairs (Fig. 5a); pseudohyphae are present. Budding is multilateral. Sediment is formed after a month, but a pellicle is not observed. After 3 days of growth on YM agar at 25 °C, colonies are white to cream-coloured, butyrous and smooth with entire margins. After 12 days at 25 °C on Dalmau plate culture on CM agar, pseudohyphae and true hyphae are formed (Fig. 5b). Sporulation occurs on 5% ME agar after 14 days at 25 °C. Unconjugated asci are formed from single cells with one elongated ascospores which are tapered and curved at the ends (Fig. 5c) Glucose and maltose are weakly fermented. Xylose fermentation is negative using Durham tubes, but ethanol is produced from xylose when determined with alcohol oxidase and peroxidase tests. Glucose, d-glucosamine, d-ribose, d-xylose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, arbutin, melezitose, inulin, ribitol, d-glucitol, d-mannitol, 2-keto-d-gluconate, succinate and ethanol are assimilated. No growth occurs with galactose, l-sorbose, l-arabinose, d-arabinose, l-rhamnose, melibiose, lactose, raffinose, glycerol, erythritol, xylitol, galactitol, myo-inositol, dglucono-1, 5-lactone, 5-keto-d-gluconate, d-gluconate, d-glucuronate, dl-lactate, citrate, l-arabinitol or methanol. For the assimilation of nitrogen compounds, growth on l-lysine, glucosamine or d-tryptophan is present, whereas growth on nitrate, nitrite, ethylamine, cadaverine, creatine, creatinine or imidazole is absent. Growth is observed at 37 °C, but not at 40 °C. Growth in the presence of 0.01% cycloheximide is present, but growth in the presence of 0.1% cycloheximide, 10% NaCl plus 5% glucose and 1% acetic acid is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Habitat: in rotting wood in Jiuxi Mountain Forest Park,

Distribution:China 

GenBank Accession: Strain NYNU 16747T\NYNU 16632 ITSMG255728\MT272352  D1/D2 MG255705\MT272350

Reference: Lv S-L, Chai C-Y, Wang Y, Yan Z-L, Hui F-L (2020) Five new additions to the genus Spathaspora (Saccharomycetales, Debaryomycetaceae) from southwest China. MycoKeys 75: 31–49. https://doi.org/10.3897/ mycokeys.75.57192

Figure 5. Morphology of Spathaspora parajiuxiensis (NYNU 16747, holotype) a budding cells and pseudohyphae on YM broth after 3 d b true hyphae with blastoconidia on CM agar after 14 d c ascus and ascospore (arrow) on 5% ME agar after 14 d. Scale bars: 10 μm.