Diaporthe jinxiu2021

Diaporthe jinxiu X.H. Wang & G.P. Wang, sp. nov.2021

MycoBank: 838502

holotype HMAS 249837, culture ex-holotype culture CGMCC3.20269 = TZFH20); ibid., ex-isotype cul ture TZFH25.

Description：

Sexual morph: Undetermined:

Asexual morph：on alfalfa stems after 15 days. Pycnidial conidiomata small, covered by pale yellow discharged conidial masses at maturity, 385–810 μm diam. Conidiophore hyaline, cylindrical, smooth, phialidic, unbranched, straight or slightly curved, 16–21 × 2–2.5 μm. Conidiogenous cells phia lidic, cylindrical. Alpha conidia hyaline, aseptate, ellipsoidal, biguttulate, rounded at each end, 5.8–7.1 × 2.7–4.0 μm (mean = 6.4 ± 0.4 × 3.5 ± 0.3 μm, n = 50). Beta and gamma conidia not observed.

Culture：Cultures incubated on PDA at 25 °C in cycle of light/ darkness, growth rate 11.5 mm per day. On PDA medium, colonies were sparse and villous, flourishing at edge of colony. On OA medium, colonies dense with neat edges, with yellow pigment in the center.

Habit：Undetermined:

Distribution：China

GenBank Accession: Undetermined:

Notes：In the phylogenetic, multi-locus tree, D. jinxiu forms a distinct clade with maximum support (1/100/99) and is most closely related to D. rhoina, but with small er pycnidial conidiomata than the later (D. jinxiu = 386–807 μm vs D. rhoina = 500 2500 μm) (Feltgen 1901). Moreover, the sequence differences were significant, and all f ive regions were able to distinguish them (28/578 in ITS, 38/382 in TEF, 21/509 in CAL, 28/520 in HIS, and 11/525 in TUB).

Reference: Undetermined

(A, B) and OA (C, D), respectively E conidiomata on alfalfa stems F conidioma G conidiophores H alpha conidia I–J section view of conidi oma. Scale bars: 2 mm (E); 200 μm (F); 20 μm (I); 10 μm (G, H, J