Diatrypella pseudooregonensis S.H. Long & Q.R. Li, sp. nov.
MycoBank No: 839656
Holotype. GMB0041
Description. Saprobic on decaying branches of unidentified plant.
Sexual morph: Stromata pustulate, with groups of 3–16 perithecia, rugose, visible as black, erumpent, scattered, surrounded by a thin, black line in host tissue, solitary to gregarious, 1–3 mm long and 0.5–2 mm broad (av. = 2 × 1.5 mm, n = 30), about 1 mm thick. Endostroma white to light yellow. Ostiole opening separately, papillate or apapillate, central. Perithecium immersed in stroma, globose to subglobose, glabrous, with cylindrical neck, brevicollous or longicollous 218.5–465 μm high, 112–257 μm diam. (av. = 306 × 164 μm, n = 10), globose to subglobose, glabrous, ostioles individual. Peridium: 30–50 μm thick, dark brown to hyaline with textura angularis cell layers. Asci 95–149 × 6.5–11.5 μm (av. = 120 × 10.5 μm, n = 30), 8-spored, unitunicate, clavate or cylindrical, long-stalked, apically rounded, apical rings inamyloid. Ascospores 11–16 × 1.5–3.5 μm (av. = 14 × 2.5 μm, n = 30), irregularly arranged, allantoid, slightly or moderately curved, subhyaline to slightly brown, smooth, aseptate, usually with two oil droplets.
Asexual morph: undetermined.
Habitat:Saprobic on decaying branches of unidentified plant.
Culture characteristics. Ascospores germinating on PDA within 24 hours. Colonies on PDA, white when young, became pale brown, dense, but thinning towards the edge, margin rough, white from above, white at margin and light brown at centre from below, no pigmentation produced on PDA medium, no conidia observed on PDA or on OA media.
GenBank Accession: GMB0041 (LSU: MW797062, RPB2: MW814906); GMB0043 (LSU: MW797064, RPB2: MW814907); GMB0040 (LSU: MW797061, RPB2: MW814905); GMB0039 (LSU: MW797059, RPB2: MW814904); GMB0042 (LSU: MW797063); GMLB0044
(LSU: MW979054, RPB2: MW814899).
Preference: Long, S., Liu, L., Pi, Y., Wu, Y., Lin, Y., Zhang, X., Long, Q., Kang, Y., Kang, J., Wijayawardene, N. N., Wang, F., Shen, X., & Li, Q. (2021). New contributions to Diatrypaceae from karst areas in China. MycoKeys, 83, 1–37. https://doi.org/10.3897/mycokeys.83.68926
Diatrypella pseudooregonensis (GMB0041, holotype) A stromata on host substrate B, C stromata on host substrate D transverse section through ascostroma E vertical section through ascostroma F culture on PDA G section through the ascostroma H ostiolar canal I, J asci K–N ascospores. Scale bars: 20 μm (G); 10 μm (H–N).
