Cryphognomonia pini C.M. Tian & N. Jiang, sp. Nov. 2020
MycoBank No: 829510
Holotype: China. Shaanxi Province: Ankang City, Huoditang forest farm, 33°26'7"N, 108°26'48"E, on branches of Pinus armandii, 8 June 2018, N. Jiang & C.M. Tian (holotype BJFC-S1725; ex-type living culture: CFCC 53020); 33°26'7"N, 108°26'48"E, on branches of Pinus armandii, 8 June 2018, N. Jiang & C.M. Tian (BJFC-S1726; living culture: CFCC 53021).
Morphological description
Pseudostromata erumpent, causing a pustulate bark surface, 650– 1200 µm diam., containing up to 12 perithecia. Central column yellowish to brownish. Stromatic zones lacking. Perithecia conspicuous, flask-shaped to spherical, umber to fuscous black, regularly scattered, 350–600 µm diam. Paraphyses deliquescent. Asci fusoid, 8-spored, biseriate, with an apical ring, (60–)65–80(–90) × (21–)22– 31(–35) µm. Ascospores hyaline, clavate to cylindrical, smooth, multi-guttulate, symmetrical to asymmetrical, straight to slightly curved, bicellular, with a median septum distinctly constricted, with distinct hyaline sheath, (15.5–)18–25(–27) × (8.5–)9.5– 11.5(–12) µm. Asexual morph: not observed.Culture characters. Cultures incubated on PDA at 25 °C in the dark, initially pale white, becoming olive-green after 3 wk. The colonies are flat, with regular margins; texture initially uniform, becoming compact after 1 month.
Habitat: on branches of Pinus armandii.
Distribution: China
GenBank Accession: ITS MK432672, MK432673; LSU MK429915, MK429916; tef1 MK578144, MK578145; rpb2 MK578100, MK578101
Notes: Cryphognomonia pini is the type species of Cryphognomonia, and occurs on Pinus armandii in China. Morphologically, Cryphognomonia pini is characterized based on bicellular ascospores with obvious hyaline sheath. In the phylogenetic tree, this species is most closely related to F. rhoigena (Fig. 1). However, Cryphognomonia pini can be distinguished from F. rhoigena based on ITS, LSU, tef1 and rpb2 loci (73/512 in ITS, 4/775 in LSU, 186/437 in tef1 and 90/1064 in rpb2).
Reference: Yang Q, Jiang N, Tian C-M (2020) Tree inhabiting gnomoniaceous species from China, with Cryphogonomonia gen. nov. proposed.
Cryphognomonia pini on Pinus armandii (BJFC-S1725) A–C habit of ascomata on twigs D, E transverse section of ascomata F longitudinal section through ascomata G asci H, I ascospores. Scale bars: 2 mm (A); 500 µm (B–F); 10 µm (G–I).
